WebJun 18, 2024 · Choose dyes with appropriate excitation wavelengths and little to no overlap in their emission spectra. Consider total fluorescence intensity as well. For example, … WebThe DAPI-FITC-TRITC triple band fluorescence filter combination is designed specifically for simultaneous detection of the fluorochromes DAPI, FITC, and TRITC with minimal crossover (spectral bleed-through) between adjacent bands, and it can also be employed with other combinations of fluorescent probes that have similar spectral profiles.The short …
Fluorescence SpectraViewer - Thermo Fisher Scientific
WebCy3 dye. Fluorescence SpectraViewer. Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 … Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally … Webemission wavelengths. Get more information on dye spectral emission and excitation values. Choose dyes that can be calibrated on your instrument. Your real-time PCR instrument must be calibrated for the set of dyes you choose (Table 1). If it is not, you must calibrate the instrument or select different dyes. IDT provides janus fund 2022 distributions
Recommended dye combinations for multiplex qPCR - Clark …
WebChannel: GL2: GL2: GL3: GL4: Compatible fluorescent proteins: tdTomato YFP: tmCherry : Antibody labels: Alexa Fluor 532 Alexa Fluor 555 DsRed Qdot 565 Qdot 605 R-phycoerythrin (R-PE, PE) Alexa Fluor 568 Alexa Fluor 594 Qdot 605 PE-Texas Red: Qdot 705 PE-Alexa Fluor 700 TRI-COLOR (PE-Cy5) PE-Cy5.5. Qdot 800 WebCy3 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). Cy3 is commonly combined with green-fluorescent dyes for double labeling. However, due to the spectral overlap the use of a narrow band-pass emission filter for ... WebInvitrogen Alexa Fluor 568 dye is a bright, orange-fluorescent dye with excitation ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. For stable signal generation in imaging and flow cytometry, Alexa Fluor 568 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability ... janus funds account