WebFeb 4, 2024 · Flavonoid biosynthesis is frequently considered unique to land plants. Some charophycean algae do tolerate terrestrial conditions ( Karsten and Holzinger, 2014; Holzinger and Pichrtová, 2016) and can produce polyphenolics in response to abiotic stresses such as UVB, salinity, or dehydration. WebApr 6, 2016 · Flavonols are a class of flavonoids that are produced by the desaturation of dihydroflavanols in a reaction that is catalyzed by flavonol synthase (FLS). In the study reported here, we cloned the full-length cDNA of FLS (designated as VcFLS) from Vaccinium corymbosum (blueberry) using rapid amplification of cDNA ends (RACE).
Regulation of flavonol biosynthesis during anther and pistil ...
WebApr 25, 2024 · The litchi gene on which SNP51 was located was annotated as flavonol synthase (FLS), which catalyzes the formation of flavonols. Here, we further elucidate the variation of the FLS gene from L. … WebJun 1, 2013 · Flavonols as an important kind of flavonoids not only have many defense-related functions, but also play an important role as potent antioxidants. Flavonol … canadian tire cordless grinder
Metabolomics and transcriptomics strategies to reveal the …
WebDec 1, 2024 · In this study, we identified a YABBY family transcription factor, AaYABBY5, as a positive regulator of anthocyanin and total flavonoid contents in A. annua. AaYABBY5 was selected based on its similar expression pattern to the phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), and flavonol synthase (FLS) … WebFlavonol synthase (FLS) is a downstream enzyme of F3H during flavonol biosynthesis. The first monocot FLS-encoding gene functionally characterized was maize ZmFLS1 with a tandemly duplicated gene … Flavonol synthase (FLS) is the key enzyme responsible for the biosynthesis of flavonols, the most abundant flavonoids, which have diverse pharmaceutical effects. Flavonol synthase has been previously found in other species, but not yet in Ornithogalum caudatum. See more After functional annotation, four unigenes, namely unigenes 61,710, 27,929, 76,508, and 101,341, showing high sequence similarity with flavonol synthase were retrieved from RNA-seq data (Additional file 1: … See more To determine the identity of these unigenes, nested PCRs were performed to isolate the corresponding cDNAs using O. caudatum cDNA as … See more Although OcFLS1 and OcFLS2 were grouped in the flavonol synthase family, comprehensive biochemical characterisation is … See more OcFLS1 and OcFLS2 were subcloned into pET-28a (+) to yield two recombinant constructs pET28a-OcFLS1 and pET28a-OcFLS2 … See more canadian tire contact number