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Hifiscript cdna

Web22 de nov. de 2024 · Total RNA was extracted with Trizon Reagent (Cwbio, China). The first-strand cDNA was prepared using the HiFiScript cDNA Synthesis kit (Cwbio, China) according to the manufacturer’s instructions. The qRT-PCR was carried out using the UltraSYBR mixture (Cwbio, China) according to the manufacturer’s protocol. WebThe iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is optimized to yield …

耐药相关基因GABRP在结直肠癌耐药中的应用【掌桥专利】

Web8 de set. de 2024 · Subsequently, HiFiScript cDNA Synthesis kit (catalog no. CW2569; CWBiotech Co. Ltd.) was used to reverse transcribe 1 µg total RNA at 37°C for 15 min and 85°C for 5 sec into cDNA. qPCR was performed using a Mastercycler ep realplex cycler with SYBR ® Green Master Mix (catalog no. CW0957 WebThis particular kit also includes 5 tubes of RNase inhibitor (200 µL each). The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions … easy college majors for athletes https://xavierfarre.com

Prognostic Profiling of the EMT-Associated and Immunity- Related ...

Web2）rnaseh活性：在反应过程中切割rna：cdna杂合链中的rna模板。降低此活性，可避免在合成较长cdna之前模板rna被降解掉。 3）dna指导的dna聚合酶活性：以反转录合成的第 … WebHIFISCRIPT 1st STRAND Cdna SYNTHESIS KIT-100 rxn- is used for the reverse transcription of double-stranded complementary DNA from mRNA, efficient, high yield … WebHiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper), including a new generation of reverse … cuprinol colours for wood

High-Capacity cDNA Reverse Transcription Kit with RNase …

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Hifiscript cdna

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WebThis product is a kit for reverse transcription after removal of genomic DNA. The kit removes genomic DNA in 2 minutes at 42°C. Meanwhile, because the reverse transcription reagents contain components that inhibit gDNA Eraser, the sample processed by gDNA Eraser can be directly used for reverse transcription reaction to synthesize cDNA.This ... Web技术领域. 本发明涉及耐药相关基因gabrp在结直肠癌耐药中的应用，属于生物医药领域。 背景技术. 结直肠癌(crc)是全球第三大常见癌症，也是第二致命的癌症，2024年约有190万例新发病例和超过935，000例死亡。

Hifiscript cdna

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WebFirst-stranded cDNA was synthesized from 4 μg RNA using a HiFiScript cDNA Synthesis kit (YEASEN, Shanghai, China) and applied as a template for qRT-PCR. Dissociation curves of qRT-PCR reaction were analyzed for the specificity of primers. qRT-PCR analysis was run on CFX96 Real Time PCR System (Bio-rad, America) using UltraSYBR mixture … Webtranscription, by applying the HiFiScript cDNA first-strand synthesis kit (CW2569M; CWBIO). We used fluorescent PCR instrument (CFX Connect™ real-time; Bio-Rad Laboratories) for performing RT-qPCR. The reaction system was: RNase Free dH2O 9.5 μL; cDNA 1 μL; upstream primer 1 μL; downstream primer 1 μL; and 2×qPCR mixture 12.5 μL.

Web18 de jul. de 2024 · HiFiScript gDNA Removal cDNA Synthesis Kit Catalog Number: CW2024S (10 rxns) CW2024M (100 rxns) Storage Condition:-20°C . Kit Components: Component CW2024S (10 rxns) CW2024M (100 rxns) gDNA Remover Mix 10 µl 100 µl 5×HiFiScript RT MasterMix 40 µl 400 µl RNase-Free Water 0.5 ml 1.5 ml Webcentration, HiFiScript cDNA Synthesis Kit (CW2569M, CWBIO, China) and miRNA cDNA Synthesis Kit (CW2141S,CWBIO,China)wereusedreversetranscription with a 20μL reverse transcription reaction system. SYBR-Green PCR Master Mix (CW2601S, CWBIO, China) was used for PCR amplification using the 30μL amplification

Web1 de fev. de 2024 · First-strand cDNA was synthesized using ProtoScript II Reverse Transcriptase and the second-strand cDNA was synthesized using Second Strand Synthesis Enzyme Mix. The bead-purified double-stranded cDNA was then treated with End Prep Enzyme Mix to repair both ends and add a dA-tailing in one reaction, followed by a … Web康为世纪生物科技股份有限公司是一家立足于生命科学领域，有自主知识产权的国家高新技术企业，主要从事高附加值、高技术含量的生物试剂的研发与生产，为生命科学研究、基 …

Web本试剂盒配有新型高效反转录酶HiFiScript，新颖突变位点大幅提升酶的转录活性，cDNA第一链合成的效率和产量更高，可利用pg级的总RNA或mRNA合成cDNA第一链。如逆转录产物cDNA用于下游荧光定量检测，可在42℃，15分钟完成逆转录反应。

Web6 de ago. de 2024 · The biological function of the aromatase gene (cyp19a) is to transform testosterone into estradiol, which is involved in ovarian differentiation and development. In the present study, in order to explore the role of cyp19a in sex differentiation of Culter alburnus, the full-length cDNA sequence was obtained, and the relationship between … easy college meals for oneWebThe kit removes genomic DNA in 2 minutes at 42°C. Meanwhile, because the reverse transcription reagents contain components that inhibit gDNA Remover, the sample … cuprinol decking paint silver birchWebHiFiScript gDNA Removal RT MasterMix GoldStar Probe mixture HiFiScript cDNA Synthesis Kit HiFiScript gDNA Removal cDNA Synthesis Kit HiFi PCR Mix for NGS MagicSYBR Mixture SARS-CoV-2 Detection Kit (qPCR Probe Assays) Bst DNA Polymerase, Large Fragment Cobuddy DNA Polymerase UltraSYBR One Step RT-qPCR … easy college schedule makerWeb18 de jul. de 2024 · HiFiScript gDNA Removal cDNA Synthesis Kit Catalog Number: CW2024S (10 rxns) CW2024M (100 rxns) Storage Condition:-20°C . Kit Components: … easy college scholarships for womenWeb11 de jan. de 2015 · 按HiFiScript 快速去基因组cDNA 第一链合成试剂盒说明将RNA 逆转录成cDNA，按2×Taq Master Mix说明进行扩增。PCR 扩增反应体系:cDNA 1 μL、上游和下游引物各1 μL、2×Master Mix 12.5 μL，ddH2O 9.5 μL，总反应体系25 μL。扩增后产物用1%琼脂糖凝胶电泳25 min 左右。 easy college scholarships 2023Web24 de mai. de 2024 · Ultrapure RNA kits (CW0581M), HiFiScript cDNA synthesis kits (CW2569M), and UltraSYBR mixture (CW0957M) were purchased from CWBIO (Beijing, China). Human A549 lung carcinoma cells were provided by Stem Cell Bank, Chinese Academy of Sciences, Shanghai, China. easy college meals on a budgetWeb丁香通为您提供RNaseOff™RNa商品详情介绍：价格：￥998，货号：CW3335M，品牌：CWBIO，保质期：12个月，详见丁香通RNaseOff™RNa商品详情页； cuprinol decking stain wickes