Webb高彩霞团队改造并比较了三组植物引导编辑系统(plant prime editor systems,PPEs),它们分别为PPE2,PPE3和PPE3b。 其中,PPE2对应上文刘如谦团队的PE2,包含融合逆转录酶M-MLV-RT的nCas9(H840A),和一个包含PBS和反转录模板的pegRNA。 PPE3对应于上文的PE3,在PPE2的基础上添加了一个可以靶向并指导切割非编辑链sgRNA。 PPE3b则 … WebbpOsU3 pTaU3 pH-nCas9-PPE-V2/pH-ePPE Forward primer (5'-3'): + Spacer sequence + Reverse primer (5'-3'): + PBS and RT template sequence + Submit >> clear. Help …
Addgene: pH-nCas9-PBE
WebbpH-nCas9-PBE DescriptionAlias:L-98163 Promoter:OsU3,UBI Replicator:pVS1 Clone strain:Stbl3 Culture conditions:37centigrade Host WebbThe pOsU3, pTaU3, and pTaU6 vectors are recommended for particle bombardment and protoplast transformation experiments. The pH-nCas9-PPE-V2 vector is recommended … sims 4 highlight skin detail
(PDF) Precise base editing in rice, wheat and maize with a Cas9 ...
Webb15 apr. 2024 · To construct the pH-nCas9 (H840A) vector for the control group, the M-MLV sequence of pH-nCas9-PPE was removed using a ClonExpress II Cloning Kit. The PE3 … WebbLocation : Home > Faculty > Caixia Gao > Selected Publications. Selected Publications. Publications (* Corresponding author; 1 co-first author) 1. Wang S1, Zong Y1, Lin Q1, Zhang H1, Chai Z, Zhang D, Chen K, Qiu JL, Gao C * (2024) Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC-Cas9. WebbPlasmid pH-nCas9-PPE-V2 from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 Mar 25. pii: 10.1038/s41587-021-00868-w. … rbwm facebook