WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb18 okt. 2024 · 载体描述:. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically …
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Webb27 mars 2024 · The Y187 yeast strains containing recombined pHIS2 vectors were first cultivated in −T−H screening medium with different concentrations of 3-amino-1,2,4-triazole (3-AT), which was used to suppress background histidine leakiness of the pHIS2 vector. The concentration of 3-AT that prevented yeast growth in −T−H screening … http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf fitty denture adhesive
Transcription Factor-Centered Yeast One-Hybrid Assay
Webb9 mars 2024 · Use the outward primers phiS2 and psiR3 together with a DNA polymerase not inhibited by. bacterial cultures such as TAQ polymerase for the PCR and sequence the insert with the nested reverse primer psiR2. Use. less than 1 l of bacterial culture supernatant per 50 l-PCR reaction or the touch of a toothpick on a colony as DNA. WebbpHIS2 Vector Information Clontech Laboratories, Inc. www.clontech.com Protocol No. PT3705-5 2 Version No. PR32049 Notice to Purchaser This product is intended to be … Webb20 dec. 2024 · GRAS transcription factor (TF) family is unique and numerous in higher plants with diverse functions that involving in plant growth and development processes, such as gibberellin (GA) signal transduction, root development, root nodule formation, and mycorrhiza formation. Walnut tree is exposed to various environmental stimulus that … fitty exercise