Phis2.1 vector

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August undefined, 2024

Webb8 juli 2024 · pHis2 编号载体名称北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体高拷贝/低拷贝: -- 启动子: -- … WebbWe'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2024 ...

pHis2_载体及菌株库_产品中心_奥诺基因-HonorGene-基因研究资 …

http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf Webb26 maj 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for … fly by night away from here

酵母单杂交.ppt-原创力文档

Webb使用方法: 1.计算所需平板的数量，计算配置培养基体积；. 2.设置3-AT的浓度梯度（建议10-50 mM 的浓度范围设3-5个梯度，常用浓度约30 mM）；. 3.灭菌筛选培养基，待培养基冷 … WebbUnique restriction sites are in bold. pHIS2.1 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was … WebbA yeast one-hybrid library was constructed to screen the binding proteins of suspensor specific cis-element. In this study, five repeats of suspensor specific cis-element … greenhouses for sale tasmania

Phis2.1 vector

WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use …

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WebbIn this construct, three copies of the DNA target (T) have been inserted into the pHIS2.1 reporter vector. GAL UAS minimal promoterADE2, HIS3, lacZ, and MEL1 transcription … Webb16 feb. 2024 · Two GA-inducible genes expansin 1 (PhEXP1) and cysteine proteinase 1 (PhCP1) were down-regulated in the flowers infected with TRV-PhCHS/GA20ox3 . …

http://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html Webb一、载体概念. 1. 载体，DNA的搬运工. 载体：在基因工程重组DNA技术中，将DNA片段（目的基因）转移至受体细胞的一种能自我复制的DNA分子。. 2. 载体，基因密码承载者. 载 …

WebbThe target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein and the target sequence stimulates … WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog No. 630304 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. …

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Webb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in … green houses for sale texashttp://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf fly by night bill hendersonWebb9 apr. 2024 · The recombinant vectors were transferred into yeast strain Y187 cells using the LiAC-PEG4000 method. The TF-encoding genes were amplified and inserted into the pGADT7 vector, and the resulting constructs were then transformed into the pHis2 yeast strain containing the promoter plasmid. fly by night birminghamWebb20 feb. 2024 · The full-length cDNA of TcbZIP60 was cloned into the pGADT7 vector used for homologous recombination, and the promoter sequences of TcCHS and TcAOC were cloned separately into the pHis2.1 vector. The resultant pGADT7: TcbZIP60 plasmid was co-transformed into yeast strain Y187, together with pHis2.1: TcCHS or pHis2.1: TcAOC , … fly by night books burlingtonhttp://www.biovector.net/product/319746.html fly by night bedsWebb19 sep. 2024 · The pHIS2 vector carries the TRP1 nutritional marker for selection in yeast. The transformed yeast cells were cultured on SD/-Trp-Leu medium and SD/-Trp-His-Leu medium supplemented with 10 mM or 30 mM 3-amino … fly by night carpets northampton maWebb16 jan. 2015 · (PR732189;publishedApril2007)pHIS2.1VectorInformationPT3951-5Sold630304RestrictionMapMultipleCloningSite(MCS)pHIS2.1Vector.Uniquerestrictionsitesbold.DescriptionpHIS2.1reportervectoryeastone … fly by night cast shannon